Rat EPX(Eosinophil Peroxidase) Microsample ELISA Kit
Biozol Catalog Number:
BYT-ORB2997344
Supplier Catalog Number:
orb2997344
Alternative Catalog Number:
BYT-ORB2997344-48,BYT-ORB2997344-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Species Reactivity:
Rat
Alternative Names:
EPO, EPP, EPX-PEN, EPER
Rat EPX(Eosinophil Peroxidase) Microsample ELISA Kit
Range:
3.13-200 ng/mL
Sensitivity:
1.3 ng/mL
Samples:
serum, plasma, tissue homogenates and other biological fluids
Application Notes:
Application Notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EPX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EPX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EPX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat EPX in the samples is then determined by comparing the OD of the samples to the standard curve
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