Human IL-2

Product Overview

• Article Name: Human IL-2
• Biozol Catalog Number: BYT-ORB3002347
• Supplier Catalog Number: orb3002347
• Alternative Catalog Number: BYT-ORB3002347-10,BYT-ORB3002347-50,BYT-ORB3002347-500,BYT-ORB3002347-1
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Alternative Names: Interleukin-2, IL-2, T-cell growth factor, TCGF, Aldesleukin

Product Description

Recombinant Human Interleukin-2 is produced by our E.coli expression system and the target gene encoding Pro22-Thr153(Cys145Ser) is expressed.

Product Properties

• Molecular Weight: Predicted: 15.5 KDa. Observed: 14 KDa, reducing conditions
• UniProt: P60568
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Form: Lyophilized from a 0.2 µm filtered solution of 10mM Acetata-Na, 5% Trehaiose, pH 4.5.

Application Notes

• Application Notes: Biological Activity: BLI: Loaded Human IL-2RA-His(Catorb2994464) on HIS1K Biosensor, can bind Human IL-2(Catorb3002347) with an affinity constant of 31 nM as determined in BLI assay. Loaded Human IL-2RA-Fc(Catorb2994463) on Protein A Biosensor, can bind Human IL-2(Catorb3002347) with an affinity constant of 13.2 nM as determined in BLI assay. Loaded Human IL-2RB-His(Catorb2994462) on HIS1K Biosensor, can bind Human IL-2(Catorb3002347) with an affinity constant of 0.19 uM as determined in BLI assay. Loaded Human IL-2RB-Fc(Catorb2994461) on Protein A Biosensor, can bind Human IL-2(Catorb3002347) with an affinity constant of 0.27 uM as determined in BLI assay. Loaded Mouse IL-2RA-His(Catorb2994413) on HIS1K Biosensor, can bind Human IL-2(Catorb3002347) with an affinity constant of 33 nM as determined in BLI assay. Loaded Biotinylated Human IL-2RB-His-Avi(Catorb2993868) on HIS1K Biosensor, can bind Human IL-2(Catorb3002347) with an affinity constant of 0.34 uM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 90-270 pg/ml