Recombinant Human Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His25-Ser153 is expressed with a human IgG1 Fc tag at the C-terminus.
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Form:
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
Application Notes:
Biological Activity: BLI: Loaded Cynomolgus IL-4RA-His(Catorb2994860) on HIS1K Biosensor, can bind Human IL-4-Fc(Catorb3002348) with an affinity constant of -3 nM as determined in BLI assay. Loaded Human IL-4RA-His(Catorb2994200) on HIS1K Biosensor, can bind Human IL-4-Fc(Catorb3002348) with an affinity constant of -3 nM as determined in BLI assay. . ELISA: Immobilized Human IL-4-Fc(Catorb3002348) at 5µg/ml (100 µl/well) can bind Cynomolgus IL-4Ra-His (Catorb2994860).The ED50 of Recombinant Human IL-4Ra-His (Catorb2994860) is 79.49 ng/ml
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