Mouse Leptin

Product Overview

• Article Name: Mouse Leptin
• Biozol Catalog Number: BYT-ORB3002378
• Supplier Catalog Number: orb3002378
• Alternative Catalog Number: BYT-ORB3002378-10,BYT-ORB3002378-50,BYT-ORB3002378-500,BYT-ORB3002378-1
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Alternative Names: Leptin, Obese Protein, Obesity Factor, LEP, OB, OBS

Product Description

Recombinant Mouse Leptin is produced by our E.coli expression system and the target gene encoding Val22-Cys167 is expressed.

Product Properties

• Molecular Weight: Predicted: 16.1 KDa. Observed: 14 KDa, reducing conditions
• UniProt: Q544U0
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Form: Lyophilized from a 0.2 µm filtered solution of 20mM PB, 300mM NaCl, pH 7.5.

Application Notes

• Application Notes: Biological Activity: BLI: Loaded Recombinant Mouse LEPR (C-10His)(Catorb2993840) on HIS1K Biosensor, can bind Recombinant Mouse Leptin(Catorb3002378) with an affinity constant of 0.196 nM as determined in BLI assay. (Regularly tested). ELISA: Immobilized Recombinant Mouse LEPR (C-10His)(Catorb2993840) at 5µg/ml (100 µl/well) can bind Recombinant Mouse Leptin(Catorb3002378)*. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin(Catorb3002378) is 1.16 ng/ml.(Regularly tested)