Recombinant Human Laminin 221 is produced by our Mammalian expression system and the target gene encoding Leu1900-Ala2722&Leu1573-Gln1798&Asn1364-Pro1609 is expressed with a 6His, Flag and HA tag at N terminus respectively.
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Form:
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
Application Notes:
Biological Activity: ELISA: Immobilized Recombinant Human Integrin alpha7X2B beta 1 at 5µg/ml (100 µl/well) can bind Recombinant Human Laminin 221 E8 Protein (Catorb3002523) *.*: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human Laminin 221 E8 Protein (Catorb3002523) is 6.06 ng/ml. (Regularly tested)
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