PSMD14 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: PSMD14 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB312510
• Supplier Catalog Number: orb312510
• Alternative Catalog Number: BYT-ORB312510-100,BYT-ORB312510-200,BYT-ORB312510-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human PSMD14 (221-310/310aa)
• Conjugation: Unconjugated
• Alternative Names: 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome regulatory subunit rpn11, 26S proteasome-associated PAD1 homolog 1, 26S proteasome-associated PAD1 homolog, PAD1, PAD1, yeast, homolog of, POH1, Proteasome(prosome, macropain) 26S subunit, non-ATPase, 14, Proteasome 26S subunit non ATPase 14, PSDE_HUMAN, Psmd14, RPN11.

Product Description

PSMD14 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 35 kDa
• UniProt: O00487
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: PSMD14

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NTF2) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PSMD14) Polyclonal Antibody, Unconjugated (orb312510) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PSMD14) Polyclonal Antibody, Unconjugated (orb312510) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PSMD14) Polyclonal Antibody, Unconjugated (orb312510) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PSMD14) Polyclonal Antibody, Unconjugated (orb312510) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PSMD14) Polyclonal Antibody, Unconjugated (orb312510) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PSMD14) Polyclonal Antibody, Unconjugated (orb312510) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: Testis (Mouse) Lysate at 40 ug Lane 3: Raw264.7 (Mouse) Lysate at 40 ug Lane 4: Testis (Rat) Lysate at 40 ug Lane 5: Cerebrum (Rat) Lysate at 40 ug Lane 6: Hela (Human) Cell Lysate at 30 ug Lane 7: U2os (Human) Cell Lysate at 30 ug Lane 8: K562 (Human) Cell Lysate at 30 ug Primary: Anti-PSMD14 (orb312510) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD.

Immunohistochemical staining of liver cancer tissue using PSMD14 antibody.