PUF60 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: PUF60 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB312531
• Supplier Catalog Number: orb312531
• Alternative Catalog Number: BYT-ORB312531-100,BYT-ORB312531-200,BYT-ORB312531-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human PUF60 (1-100/559aa)
• Conjugation: Unconjugated
• Alternative Names: 60 kDa poly(U)-binding-splicing factor, FBP-interacting repressor, FIR, FLJ31379, FUSE-binding protein-interacting repressor, Poly U binding splicing factor 60KDa, Poly(U)-binding-splicing factor PUF60, poly-U binding splicing factor PUF60, PUF60, PUF60_HUMAN, pyrimidine tract binding splicing factor, Ro ribonucleoprotein-binding protein 1, Ro-binding protein 1, RoBP1, RoBPI, siah binding protein 1, Siah-binding protein 1, Siah-BP1, SIAHBP1.

Product Description

PUF60 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 60 kDa
• UniProt: Q9UHX1
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: PUF60

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test

Images

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-PUF60 antibody (orb312531), dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PUF60) Polyclonal Antibody, Unconjugated (orb312531) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PUF60) Polyclonal Antibody, Unconjugated (orb312531) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PUF60) Polyclonal Antibody, Unconjugated (orb312531) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PUF60) Polyclonal Antibody, Unconjugated (orb312531) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-PUF60 (orb312531) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-PUF60 (orb312531) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 63 kD.

Sample: LOVO (Human) Cell Lysate at 30 ug, Primary:Anti-PUF60 (orb312531) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 64 kD.

Flow cytometry of Hela.Primary Antibody (green line): Rabbit Anti-PUF60 antibody, Dilution:2ug/Test.