Simian pMAPT /pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit
Biozol Catalog Number:
BYT-ORB3126911
Supplier Catalog Number:
orb3126911
Alternative Catalog Number:
BYT-ORB3126911-48, BYT-ORB3126911-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Species Reactivity:
Primate
Alternative Names:
pMAPT /pTAU
Simian pMAPT /pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit
Range:
31.25-2000 pg/mL
Sensitivity:
12.98 pg/mL
Samples:
serum, plasma, other biological fluids
Application Notes:
Application Notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian pMAPT /pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian pMAPT /pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian pMAPT /pTAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Simian pMAPT /pTAU in the samples is then determined by comparing the OD of the samples to the standard curve
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