Simian IL1h(Interleukin 1 Eta) ELISA Kit

Catalog Number: BYT-ORB3127009
Article Name: Simian IL1h(Interleukin 1 Eta) ELISA Kit
Biozol Catalog Number: BYT-ORB3127009
Supplier Catalog Number: orb3127009
Alternative Catalog Number: BYT-ORB3127009-48, BYT-ORB3127009-96
Manufacturer: Biorbyt
Category: Kits/Assays
Species Reactivity: Primate
Alternative Names: IL1F8, IL36b, FIL1, FIL1-(ETA), FIL1H, IL-1F8, IL-1H2, IL1-ETA, IL1H2, Interleukin 36, Beta, Interleukin-1 Family Member 8, Interleukin-1 homolog 2
Simian IL1h(Interleukin 1 Eta) ELISA Kit
Range: 7.82-500 pg/mL
Sensitivity: 2.6 pg/mL
Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes: Application Notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian IL1h. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian IL1h. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian IL1h, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Simian IL1h in the samples is then determined by comparing the OD of the samples to the standard curve