ODDD, GJAL, GJA1, GJ-A1, Gap junction 43 kDa heart protein, Gap Junction Protein Alpha 1, Oculodentodigital Dysplasia, Syndactyly Type III
Simian CX43(Connexin 43) ELISA Kit
Range:
0.32-20 ng/mL
Sensitivity:
0.121 ng/mL
Samples:
tissue homogenates, cell lysates and other biological fluids
Application Notes:
Application Notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian CX43. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian CX43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian CX43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Simian CX43 in the samples is then determined by comparing the OD of the samples to the standard curve
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