Simian PIIINP(Procollagen III N-Terminal Propeptide) ELISA Kit
Biozol Catalog Number:
BYT-ORB3127057
Supplier Catalog Number:
orb3127057
Alternative Catalog Number:
BYT-ORB3127057-48, BYT-ORB3127057-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Species Reactivity:
Primate
Alternative Names:
P3NP, N-Propeptide Of Type III Procollagen, Procollagen III Amino Terminal Propeptide
Simian PIIINP(Procollagen III N-Terminal Propeptide) ELISA Kit
Range:
6.25-400 ng/mL
Sensitivity:
2.19 ng/mL
Samples:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Application Notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian PIIINP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian PIIINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian PIIINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Simian PIIINP in the samples is then determined by comparing the OD of the samples to the standard curve
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