0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
SNRPE
Application Dilute:
IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (mouse embryo), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SNRPE) Polyclonal Antibody, Unconjugated (orb313016) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Tissue/Cell: mouse embryos tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-SNRPE Polyclonal Antibody, Unconjugated (orb313016) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical staining of mouse embryos tissue using SNRPE antibody.
Immunohistochemical staining of mouse skin tissue using SNRPE antibody.
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