Phospho-Src (Ser75) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-Src (Ser75) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB313108
• Supplier Catalog Number: orb313108
• Alternative Catalog Number: BYT-ORB313108-100, BYT-ORB313108-200, BYT-ORB313108-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Src around the phosphorylation site of Ser75 VT(p-S)PQ
• Conjugation: Unconjugated
• Alternative Names: Src (p-S75), p-Src, phospho-Src, ASV, SRC1, THC6, c-SRC, p60-Src, pp60c-src, SRC_HUMAN, SRC, Proto-oncogene c-Src, pp60c-src (p60-Src), 2.7.10.2, SRC_MOUSE, SRC_RAT,

Product Description

Phospho-Src (Ser75) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 61 kDa
• UniProt: P12931
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: SRC

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-800
• Application Notes: Modification: Phosphorylated

Images

Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Src (Ser75)) Polyclonal Antibody, Unconjugated (orb313108) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Src (Ser75)) Polyclonal Antibody, Unconjugated (orb313108) at 1:400 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868589) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Src (Ser75)) Polyclonal Antibody, Unconjugated (orb313108) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Src (Ser75)) Polyclonal Antibody, Unconjugated (orb313108) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Src (Ser75)) Polyclonal Antibody, Unconjugated (orb313108) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Src (Ser75)) Polyclonal Antibody, Unconjugated (orb313108) at 1:400 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868589) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Src (Ser75)) Polyclonal Antibody, Unconjugated (orb313108) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Src (Ser75)) Polyclonal Antibody, Unconjugated (orb313108) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation

IHC-P analysis of rat uterus using phospho-Src Ser75 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of mouse skin using phospho-Src Ser75 Polyclonal Antibody at 1:200 dilution.