SMARCA5 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: SMARCA5 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB313175
• Supplier Catalog Number: orb313175
• Alternative Catalog Number: BYT-ORB313175-100, BYT-ORB313175-200, BYT-ORB313175-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human SMARCA5 (901-1000/1052aa)
• Conjugation: Unconjugated
• Alternative Names: ISWI, SNF2H, WCRF135, hISWI, hSNF2H, SMCA5_HUMAN, SMARCA5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5, Sucrose nonfermenting protein 2 homolog (hSNF2H), 3.6.4.-,

Product Description

SMARCA5 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 122 kDa
• UniProt: O60264
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: SMARCA5

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SNF2H) Polyclonal Antibody, Unconjugated (orb313175) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SNF2H) Polyclonal Antibody, Unconjugated (orb313175) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Primary: Anti-SNF2H (orb313175) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 122 kDa, Observed band size: 130 kDa.

Sample: Panc-1 (Human) Cell Lysate at 30 ug, Primary: Anti-SNF2H (orb313175) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 122 kD, Observed band size: 127 kD.

Western blot:Panc-1(Human) Cell,Primary:Anti-SNF2H at 1:1000 dilution.

Western blot:Lane 1:Mouse Testis,Lane 2:Rat Testis,Primary:Anti-SNF2H at 1:1000 dilution.