TREM2 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: TREM2 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB313249
• Supplier Catalog Number: orb313249
• Alternative Catalog Number: BYT-ORB313249-100,BYT-ORB313249-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human TREM2
• Conjugation: Unconjugated
• Alternative Names: Triggering receptor expressed on myeloid cells 2, TREM-2, TREM2a, TREM2b, TREM2c, Trggering receptor expressed on myeloid cells 2a, TREM2_HUMAN.

Product Description

TREM2 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 35-40 kDa
• Pubmed: 35781003
• UniProt: Q9NZC2
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: TREM2

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200

Images

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated (orb313249) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated (orb313249) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated (orb313249) at 1:400 overnight at 4C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Lane 1: Mouse Raw264.7 cell lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Mouse Cerebrum tissue lysates, Lane 4: Mouse BV2 cell lysates, Lane 5: Mouse Lung tissue lysates, Lane 6: Rat Spleen tissue lysates, Lane 7: Rat Cerebrum tissue lysates, Primary: Anti-TREM2 (orb313249) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kDa, Observed band size: 24 kDa.

Sample: Lane 1: Mouse Spleen tissue lysates, Lane 2: Mouse Raw264.7 cell lysates, Lane 3: Mouse BV2 cell lysates, Lane 4: Human HepG2 cell lysates, Primary: Anti-TREM2 (orb313249) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kDa, Observed band size: 26 kDa.

Western blot analysis of MCF-7(Human) Cell Lysate & Raw264.7(Mouse) Cell Lysate using TREM2 antibody

Western blot analysis of MCF-7 Cell (Human) Lysate using TREM2 antibody.

Western blot analysis of Raji cell lysate using TREM2 antibody.