EliKine Human EGF ELISA Kit

Product Overview

• Article Name: EliKine Human EGF ELISA Kit
• Biozol Catalog Number: BYT-ORB3145520
• Supplier Catalog Number: orb3145520
• Alternative Catalog Number: BYT-ORB3145520-48, BYT-ORB3145520-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: Beta urogastrone, Epidermal growth factor, HOMG4, OTTHUMP00000219721, OTTHUMP00000219722, Pro epidermal growth factor, URG, Urogastrone

Product Description

EliKine Human EGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human EGF.

Product Properties

• Range: 3.9 pg/mL-250 pg/mL
• Sensitivity: 2 pg/Ml
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: Application Notes: EliKine Human EGF ELISA Kit employs a two-site sandwich ELISA to quantitate EGF in samples. An antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGF is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGF bound in the initial step. The color development is stopped and the intensity of the color is measured