EliKine Human bFGF ELISA Kit

Product Overview

• Article Name: EliKine Human bFGF ELISA Kit
• Biozol Catalog Number: BYT-ORB3145521
• Supplier Catalog Number: orb3145521
• Alternative Catalog Number: BYT-ORB3145521-48, BYT-ORB3145521-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: Basic fibroblast growth factor, BFGF, FGF 2, FGF B, FGF2, FGF2 basic, FGFB, Fibroblast growth factor 2 (basic), HBGF 2, HBGF2, HBGH 2, Heparin binding growth, factor 2, Heparin binding growth factor 2 precursor, OTTMUSP00000046776, Prostatropin

Product Description

EliKine Human bFGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human bFGF.

Product Properties

• Range: 15.6 pg/mL-1000 pg/mL
• Sensitivity: 8 pg/mL
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: Application Notes: EliKine Human bFGF ELISA Kit employs a two-site sandwich ELISA to quantitate bFGF in samples. An antibody specific for bFGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any bFGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for bFGF is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of bFGF bound in the initial step. The color development is stopped and the intensity of the color is measured