EliKine Human G-CSF ELISA Kit

Catalog Number: BYT-ORB3145522
Article Name: EliKine Human G-CSF ELISA Kit
Biozol Catalog Number: BYT-ORB3145522
Supplier Catalog Number: orb3145522
Alternative Catalog Number: BYT-ORB3145522-48, BYT-ORB3145522-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: C17orf33, Colony stimulating factor 3 (granulocyte), CSF 3, CSF beta, CSF3, CSF3OS, Csfg, Filgrastim, GCSA, GCSF, Granulocyte colony stimulating fact, Lenograstim, Macrophage granulocyte inducer 2
EliKine Human G-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate Human G-CSF.
Range: 31.25 pg/mL-2000 pg/mL
Sensitivity: 16 pg/mL
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: EliKine Human G-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate G-CSF in samples. An antibody specific for G-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any G-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for G-CSF is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured