EliKine Human GM-CSF ELISA Kit

Catalog Number: BYT-ORB3145523
Article Name: EliKine Human GM-CSF ELISA Kit
Biozol Catalog Number: BYT-ORB3145523
Supplier Catalog Number: orb3145523
Alternative Catalog Number: BYT-ORB3145523-48, BYT-ORB3145523-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: GM-CSF, GMCSF, Colony stimulating factor, Granulocyte Macrophage Colony Stimulating Factor, CSF 2, CSF2
EliKine Human GM-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate Human GM-CSF.
Range: 11.72 pg/mL-750 pg/mL
Sensitivity: 5 pg/mL
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: EliKine Human GM-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate GM-CSF in samples. An antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GM-CSF is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured