EliKine Human HGF ELISA Kit

Product Overview

• Article Name: EliKine Human HGF ELISA Kit
• Biozol Catalog Number: BYT-ORB3145524
• Supplier Catalog Number: orb3145524
• Alternative Catalog Number: BYT-ORB3145524-48, BYT-ORB3145524-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: DFNB39, F TCF, Fibroblast derived tumor cytotoxic factor, Hepatocyte growth factor, Hepatopoietin A, Hepatopoietin-A, Hgf, HGFB, HPTA

Product Description

EliKine Human HGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human HGF.

Product Properties

• Range: 125 pg/mL-8000 pg/mL
• Sensitivity: 60 pg/mL
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: Application Notes: EliKine Human HGF ELISA Kit employs a two-site sandwich ELISA to quantitate HGF in samples. An antibody specific for HGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGF is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGF bound in the initial step. The color development is stopped and the intensity of the color is measured