EliKine Human IL-18 ELISA Kit

Catalog Number: BYT-ORB3145538
Article Name: EliKine Human IL-18 ELISA Kit
Biozol Catalog Number: BYT-ORB3145538
Supplier Catalog Number: orb3145538
Alternative Catalog Number: BYT-ORB3145538-48, BYT-ORB3145538-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: IL-18, IL18, IL 18, IFN gamma inducing factor, Interleukin 18, Interleukin-18, Interleukin 18 (interferon-gamma-inducing factor)
EliKine Human IL-18 ELISA Kit employs a two-site sandwich ELISA to quantitate Human IL-18.
Range: 156 pg/mL- 10000 pg/mL
Sensitivity: 40 pg/mL
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: EliKine Human IL-18 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-18 in samples. An antibody specific for IL-18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-18 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-18 bound in the initial step. The color development is stopped and the intensity of the color is measured