EliKine Human Leptin ELISA Kit

Product Overview

• Article Name: EliKine Human Leptin ELISA Kit
• Biozol Catalog Number: BYT-ORB3145541
• Supplier Catalog Number: orb3145541
• Alternative Catalog Number: BYT-ORB3145541-48, BYT-ORB3145541-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: FLJ94114, LEP, LEP_HUMAN, LEPD, Leptin, Leptin Precursor Obesity Factor, OB, Obese protein

Product Description

EliKine Human Leptin ELISA Kit employs a two-site sandwich ELISA to quantitate Human Leptin .

Product Properties

• Range: 31.25 pg/mL- 2000 pg/mL
• Sensitivity: 16 pg/mL
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: Application Notes: EliKine Human Leptin ELISA Kit employs a two-site sandwich ELISA to quantitate Leptin in samples. An antibody specific for Leptin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Leptin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Leptin is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound in the initial step. The color development is stopped and the intensity of the color is measured