EliKine Human MMP-9 ELISA Kit

Catalog Number: BYT-ORB3145542
Article Name: EliKine Human MMP-9 ELISA Kit
Biozol Catalog Number: BYT-ORB3145542
Supplier Catalog Number: orb3145542
Alternative Catalog Number: BYT-ORB3145542-48, BYT-ORB3145542-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: MMP 9, MMP-9, MMP9, Matrix Metalloproteinase 9, GELB, Type V collagenase, Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
EliKine Human MMP-9 ELISA Kit employs a two-site sandwich ELISA to quantitate Human MMP-9.
Range: 31.25 pg/mL- 2000 pg/mL
Sensitivity: 16 pg/mL
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: EliKine Human MMP-9 ELISA Kit employs a two-site sandwich ELISA to quantitate MMP-9 in samples. An antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-9 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound in the initial step. The color development is stopped and the intensity of the color is measured