Application Notes: EliKine Human Resistin ELISA Kit employs a two-site sandwich ELISA to quantitate Resistin in samples. An antibody specific for Resistin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Resistin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Resistin is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Resistin bound in the initial step. The color development is stopped and the intensity of the color is measured
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