EliKine Human TGF-beta1 ELISA Kit

Catalog Number: BYT-ORB3145545
Article Name: EliKine Human TGF-beta1 ELISA Kit
Biozol Catalog Number: BYT-ORB3145545
Supplier Catalog Number: orb3145545
Alternative Catalog Number: BYT-ORB3145545-48, BYT-ORB3145545-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human, Mouse, Rat
Alternative Names: TGFB1, CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 protein, latency-associated peptide
EliKine Human TGF-beta1 ELISA Kit employs a two-site sandwich ELISA to quantitate Human TGF-beta1.
Range: 15.6 pg/ml-1000 pg/ml
Sensitivity: 8 pg/mL
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: EliKine Human TGF-beta1 ELISA Kit employs a two-site sandwich ELISA to quantitate TGF-beta1 in samples. An antibody specific for TGF-beta1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-beta1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-beta1 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-beta1 bound in the initial step. The color development is stopped and the intensity of the color is measured