EliKine Human VEGF ELISA Kit

Product Overview

• Article Name: EliKine Human VEGF ELISA Kit
• Biozol Catalog Number: BYT-ORB3145548
• Supplier Catalog Number: orb3145548
• Alternative Catalog Number: BYT-ORB3145548-48, BYT-ORB3145548-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: VEGFA, RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165, vascular permeability factor

Product Description

EliKine Human VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human VEGF-A.

Product Properties

• Range: 31.25 pg/mL-2000 pg/mL
• Sensitivity: 16 pg/mL
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: Application Notes: EliKine Human VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF-A in samples. An antibody specific for VEGF-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF-A is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound in the initial step. The color development is stopped and the intensity of the color is measured