EliKine Mouse CCL2 ELISA Kit

Product Overview

• Article Name: EliKine Mouse CCL2 ELISA Kit
• Biozol Catalog Number: BYT-ORB3145550
• Supplier Catalog Number: orb3145550
• Alternative Catalog Number: BYT-ORB3145550-48, BYT-ORB3145550-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Mouse
• Alternative Names: CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF, monocyte chemoattractant protein-1, monocyte chemotactic and activating factor, monocyte secretory protein JE, small inducible cytokine

Product Description

EliKine Mouse CCL2 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse CCL2.

Product Properties

• Range: 31.25 pg/mL-2000 pg/mL
• Sensitivity: 16 pg/mL
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: Application Notes: EliKine Mouse CCL2 ELISA Kit employs a two-site sandwich ELISA to quantitate CCL2 in samples. An antibody specific for CCL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL2 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL2 bound in the initial step. The color development is stopped and the intensity of the color is measured