EliKine Mouse IL-1alpha ELISA Kit

Product Overview

• Article Name: EliKine Mouse IL-1alpha ELISA Kit
• Biozol Catalog Number: BYT-ORB3145553
• Supplier Catalog Number: orb3145553
• Alternative Catalog Number: BYT-ORB3145553-48, BYT-ORB3145553-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Mouse
• Alternative Names: Hematopoietin 1, IL 1 alpha, Il 1a, IL1, IL1 ALPHA, IL1A, IL1F1, Ilia, Interleukin 1 alpha, Interleukin 1 alpha precursor, Interleukin1 alpha

Product Description

EliKine Mouse IL-1alpha ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-1alpha.

Product Properties

• Range: 7.8 pg/mL-500 pg/mL
• Sensitivity: 4 pg/mL
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: Application Notes: EliKine Mouse IL-1alpha ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1alpha in samples. An antibody specific for IL-1alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1alpha is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1alpha bound in the initial step. The color development is stopped and the intensity of the color is measured