EliKine Mouse IL-1beta ELISA Kit

Product Overview

• Article Name: EliKine Mouse IL-1beta ELISA Kit
• Biozol Catalog Number: BYT-ORB3145554
• Supplier Catalog Number: orb3145554
• Alternative Catalog Number: BYT-ORB3145554-48, BYT-ORB3145554-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Mouse
• Alternative Names: IL1B, IL-1, IL1-BETA, IL1F2, catabolin, preinterleukin 1 beta, pro-interleukin-1-beta

Product Description

EliKine Mouse IL-1beta ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-1beta.

Product Properties

• Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: 8 pg/mL
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: Application Notes: EliKine Mouse IL-1beta ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1beta in samples. An antibody specific for IL-1beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1beta is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1beta bound in the initial step. The color development is stopped and the intensity of the color is measured