EliKine Rat IL-2 ELISA Kit

Catalog Number: BYT-ORB3145569
Article Name: EliKine Rat IL-2 ELISA Kit
Biozol Catalog Number: BYT-ORB3145569
Supplier Catalog Number: orb3145569
Alternative Catalog Number: BYT-ORB3145569-48, BYT-ORB3145569-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Rat
Alternative Names: IL 2, IL2, IL-2, TCGF, lymphokine, T cell growth factor, aldesleukin, interleukin-2, involved in regulation of T-cell clonal expansion
EliKine Rat IL-2 ELISA Kit employs a two-site sandwich ELISA to quantitate Rat IL-2.
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: EliKine Rat IL-2 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-2 in samples. An antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-2 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound in the initial step. The color development is stopped and the intensity of the color is measured