EliKine Rat VEGF ELISA Kit

Product Overview

• Article Name: EliKine Rat VEGF ELISA Kit
• Biozol Catalog Number: BYT-ORB3145575
• Supplier Catalog Number: orb3145575
• Alternative Catalog Number: BYT-ORB3145575-48, BYT-ORB3145575-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Rat
• Alternative Names: VEGFA, RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165, vascular permeability factor

Product Description

EliKine Rat VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate Rat VEGF.

Product Properties

• Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: 8 pg/mL
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: Application Notes: EliKine Rat VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF in samples. An antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured