EliKine Human beta-hCG ELISA Kit employs a two-site sandwich ELISA to quantitate Human beta-hCG.
Range:
8 IU/L-240 IU/L
Sensitivity:
2.0 IU/L
Samples:
SerumPlasmaOther biological fluids
Application Notes:
Application Notes: EliKine Human beta-hCG ELISA Kit employs a two-site sandwich ELISA to quantitate beta-hCG in samples. An antibody specific for beta-hCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-hCG present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for beta-hCG is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-hCG bound in the initial step. The color development is stopped and the intensity of the color is measured
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