Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit

Catalog Number: BYT-ORB3145825
Article Name: Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit
Biozol Catalog Number: BYT-ORB3145825
Supplier Catalog Number: orb3145825
Alternative Catalog Number: BYT-ORB3145825-48,BYT-ORB3145825-96,BYT-ORB3145825-5,BYT-ORB3145825-50
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: UBE3A, ANCR, AS, E6-AP, EPVE6AP, FLJ26981, HPVE6A, CTCL tumor antigen se37-2, human papilloma virus E6-associated protein, oncogenic protein-associated protein E6-AP
This Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE3A.
Range: Please inquire
Sensitivity: Please inquire
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: This Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE3A in samples. An antibody specific for UBE3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE3A bound in the initial step. The color development is stopped and the intensity of the color is measured