Human Ubiquitin-conjugating enzyme E2 L3 (UBE2L3) ELISA Kit

Catalog Number: BYT-ORB3145826
Article Name: Human Ubiquitin-conjugating enzyme E2 L3 (UBE2L3) ELISA Kit
Biozol Catalog Number: BYT-ORB3145826
Supplier Catalog Number: orb3145826
Alternative Catalog Number: BYT-ORB3145826-48,BYT-ORB3145826-96,BYT-ORB3145826-5,BYT-ORB3145826-50
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: UBE2L3, E2-F1, L-UBC, UBCH7, UbcM4, ubiquitin carrier protein, ubiquitin-conjugating enzyme UBCH7, ubiquitin-protein ligase
This Human Ubiquitin-conjugating enzyme E2 L3 (UBE2L3) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2L3.
Range: Please inquire
Sensitivity: Please inquire
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: This Human Ubiquitin-conjugating enzyme E2 L3 (UBE2L3) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2L3 in samples. An antibody specific for UBE2L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2L3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2L3 bound in the initial step. The color development is stopped and the intensity of the color is measured