This Human B cell activation factor from the tumor necrosis factor family receptor (BAFF-R) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF13C.
Application Notes: This Human B cell activation factor from the tumor necrosis factor family receptor (BAFF-R) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF13C in samples. An antibody specific for TNFRSF13C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF13C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF13C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF13C bound in the initial step. The color development is stopped and the intensity of the color is measured
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