Human Kinetochore protein Spc25 (SPC25) ELISA Kit

Catalog Number: BYT-ORB3146260
Article Name: Human Kinetochore protein Spc25 (SPC25) ELISA Kit
Biozol Catalog Number: BYT-ORB3146260
Supplier Catalog Number: orb3146260
Alternative Catalog Number: BYT-ORB3146260-48, BYT-ORB3146260-5, BYT-ORB3146260-50, BYT-ORB3146260-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: SPC25, AD024, MGC22228, SPBC25, 2600017H08Rik, kinetochore protein Spc25, spindle pole body component 25, spindle pole body component 25 homolog
This Human Kinetochore protein Spc25 (SPC25) ELISA Kit employs a two-site sandwich ELISA to quantitate SPC25.
Range: Please inquire
Sensitivity: Please inquire
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: This Human Kinetochore protein Spc25 (SPC25) ELISA Kit employs a two-site sandwich ELISA to quantitate SPC25 in samples. An antibody specific for SPC25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPC25 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPC25 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPC25 bound in the initial step. The color development is stopped and the intensity of the color is measured