Human Mothers against decapentaplegic homolog 9 (SMAD9) ELISA Kit

Catalog Number: BYT-ORB3146294
Article Name: Human Mothers against decapentaplegic homolog 9 (SMAD9) ELISA Kit
Biozol Catalog Number: BYT-ORB3146294
Supplier Catalog Number: orb3146294
Alternative Catalog Number: BYT-ORB3146294-48, BYT-ORB3146294-5, BYT-ORB3146294-50, BYT-ORB3146294-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: SMAD9, MADH6, MADH9, SMAD8, SMAD8A, SMAD8B, MAD (mothers against decapentaplegic, Drosophila) homolog 9, MAD, mothers against decapentaplegic homolog 9, Mothers against decapentaplegic, drosophila, homolog o
This Human Mothers against decapentaplegic homolog 9 (SMAD9) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD9.
Range: Please inquire
Sensitivity: Please inquire
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: This Human Mothers against decapentaplegic homolog 9 (SMAD9) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD9 in samples. An antibody specific for SMAD9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD9 bound in the initial step. The color development is stopped and the intensity of the color is measured