Human CAMP-dependent protein kinase type I-beta regulatory subunit (PRKAR1B) ELISA Kit

Catalog Number: BYT-ORB3146875
Article Name: Human CAMP-dependent protein kinase type I-beta regulatory subunit (PRKAR1B) ELISA Kit
Biozol Catalog Number: BYT-ORB3146875
Supplier Catalog Number: orb3146875
Alternative Catalog Number: BYT-ORB3146875-48, BYT-ORB3146875-5, BYT-ORB3146875-50, BYT-ORB3146875-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: PRKAR1B, PRKAR1, OTTHUMP00000199948, OTTHUMP00000199949
This Human CAMP-dependent protein kinase type I-beta regulatory subunit (PRKAR1B) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR1B.
Range: Please inquire
Sensitivity: Please inquire
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: This Human CAMP-dependent protein kinase type I-beta regulatory subunit (PRKAR1B) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR1B in samples. An antibody specific for PRKAR1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR1B bound in the initial step. The color development is stopped and the intensity of the color is measured