PRIM1, MGC12308, p49, DNA primase 1, DNA primase polypeptide 1, DNA primase small subunit, DNA primase subunit 48, primase p49 subunit, primase polypeptide 1, 49kDa
This Human DNA primase small subunit (PRIM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRIM1.
Application Notes: This Human DNA primase small subunit (PRIM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRIM1 in samples. An antibody specific for PRIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRIM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRIM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRIM1 bound in the initial step. The color development is stopped and the intensity of the color is measured
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