Human Perforin-1 (PRF1/PFP) ELISA Kit

Catalog Number: BYT-ORB3146892
Article Name: Human Perforin-1 (PRF1/PFP) ELISA Kit
Biozol Catalog Number: BYT-ORB3146892
Supplier Catalog Number: orb3146892
Alternative Catalog Number: BYT-ORB3146892-48, BYT-ORB3146892-5, BYT-ORB3146892-50, BYT-ORB3146892-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: PRF1, RP11-710A11.3, FLH2, HPLH2, MGC65093, P1, PFN1, PFP, OTTHUMP00000019759, cytolysin, lymphocyte pore forming protein, perforin 1
This Human Perforin-1 (PRF1/PFP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRF1.
Range: Please inquire
Sensitivity: Please inquire
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: Application Notes: This Human Perforin-1 (PRF1/PFP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRF1 in samples. An antibody specific for PRF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRF1 bound in the initial step. The color development is stopped and the intensity of the color is measured