Application Notes: This Human Phosphoglycolate phosphatase (PGP) ELISA Kit employs a two-site sandwich ELISA to quantitate PGP in samples. An antibody specific for PGP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGP bound in the initial step. The color development is stopped and the intensity of the color is measured
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