Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) ELISA Kit

Product Overview

• Article Name: Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) ELISA Kit
• Biozol Catalog Number: BYT-ORB3147017
• Supplier Catalog Number: orb3147017
• Alternative Catalog Number: BYT-ORB3147017-48, BYT-ORB3147017-5, BYT-ORB3147017-50, BYT-ORB3147017-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: PCK2, PEPCK, PEPCK-M, PEPCK2, PEP carboxykinase, mitochondrial phosphoenolpyruvate carboxykinase 2, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase

Product Description

This Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCK2.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: Application Notes: This Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCK2 in samples. An antibody specific for PCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured