Creatine is a nitrogen-containing organic acid primarily stored in the muscles and brain of vertebrates, where it participates in energy metabolism (facilitating rapid ATP generation via phosphocreatine). The levels of creatine in animals are influenced by factors such as diet, physical activity, breed, age, and health status. CheKine Micro Creatine Content Assay Kit provides a simple, convenient, and rapid approach for measuring creatine levels, suitable for animal tissue and serum (plasma) samples. The assay is based on the reaction of creatine with diacetyl-alpha-naphthol under alkaline conditions, forming a red-colored product with an absorption peak at 520 nm.
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