KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y693 of human STAT4 protein. The exact sequence is proprietary.
Conjugation:
Unconjugated
Alternative Names:
Signal transducer and activator of transcription 4
The STAT4 (Phospho-Y693) Antibody is suitable for IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y693 of human STAT4 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Target:
STAT4
Application Dilute:
WB: 1-500:1000, IHC-P: 1-100:200, IP: 1-10:100
Application Notes:
Modification: Phosphorylated
Western blot analysis of STAT4 (Phospho-Y693) expression in HEK293T (A), PC3 (B) whole cell lysates. (Predicted band size: 85 kD, Observed band size: 95 kD)
Immunohistochemical analysis of STAT4 (Phospho-Y693) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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