The LKB1 (Phospho-T189) Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T189 of human LKB1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Target:
STK11
Application Dilute:
WB: 1-500:1000
Application Notes:
Modification: Phosphorylated
Western blot analysis of LKB1 (Phospho-T189) expression in AML12 (A), PC12 (B), A375 (C), Panc1 (D), SHSY5Y (E) whole cell lysates. (Predicted band size: 48 kD, Observed band size: 54 kD)
Immunohistochemical analysis of LKB1 (Phospho-T189) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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