SARS-CoV-2 Spike Trimer Protein, His Tag (KP.3) (MALS verified), Unconjugated

Product Overview

• Article Name: SARS-CoV-2 Spike Trimer Protein, His Tag (KP.3) (MALS verified), Unconjugated
• Biozol Catalog Number: BYT-ORB3158198
• Supplier Catalog Number: orb3158198
• Alternative Catalog Number: BYT-ORB3158198-1,BYT-ORB3158198-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Species Reactivity: Virus
• Conjugation: Unconjugated
• Alternative Names: sars-cov-2

Product Description

SARS-CoV-2 Spike Trimer Protein, His Tag (KP.3) (SPN-S522e) is expressed from human 293 cells (HEK293). It contains AA Val 16 -Pro 1213 (Accession QHD43416.1 (T19I, R21T, LPP24-26Del, A27S, S50L, H69del, V70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478K, N481K, V483del, E484K, F486P, Q493E, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, N764K, D796Y, S939F, Q954H, N969K, V1104L, P1143L, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Product Properties

• Molecular Weight: 137.7 kDa
• NCBI: 264
• Buffer: 0.1 M Sodium citrate, pH 5.5
• Purity: 90%
• Form: Powder
• Target: Spike protein

Application Notes

• Application Notes: Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 137.7 kDa. The protein migrates as 150-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation