Application Notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 16.8 kDa. The protein migrates as 20-23 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
SDS-PAGE analysis of Human IL-17F protein
Loaded Human IL-17 RA, Fc Tag on Protein A Biosensor, can bind Human IL-17F (H161R), His Tag (Cat. No. orb334906) with an affinity constant of 299 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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