Mouse IgG2a isotype Control Antibody, Clone: [M2A], Unconjugated
Biozol Catalog Number:
BYT-ORB343777
Supplier Catalog Number:
orb343777
Alternative Catalog Number:
BYT-ORB343777-1
Manufacturer:
Biorbyt
Category:
Proteine/Peptide
Application:
FC, SDS-PAGE
Conjugation:
Unconjugated
Alternative Names:
MOUSE IgG2a isotype Control
Mouse IgG2a isotype Control Antibody
Concentration:
1.0 mg/mL
Clone Designation:
[M2A]
Isotype:
IgG2a
Buffer:
0.01% (w/v) Sodium Azide
Source:
Mouse
Purity:
Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Confirmed by SDS-PAGE.
Form:
Liquid
Application Dilute:
FC: 1:1000-1:5000
Application Notes:
Biological Origin: Mouse. Application Notes: Mouse IgG2a isotype control has been tested by SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important
(E) Cell surface-bound gp130 was down-regulated in OVCAR-8 by SC144 at the indicated concentration and time. After treatment, cells were collected and stained with anti-gp130 (B-R3) antibody and analyzed by FACS. Upper panel, one of three representative experiments. Lower panels, statistical analyses of data from the three experimental repeats. Error bars = SEM. * P < 0.05, ** P < 0.01, paired t test. Mouse IgG2a (p/n orb343777).
SDS-Page Results of Mouse IgG2a Isotype Control. Lane 1: Mouse IgG2a Isotype Control Reduced [1.0 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Mouse IgG2a Isotype Control Non-Reduced [1.0 µg]. 4-20% gel. Coomassie stained.
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