RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rat IgG, and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Rat IgG1 confirmed by Dot Blot.
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: 1:2000 - 1:20,000, FC: 1:1000-1:5000
Application Notes:
Biological Origin: Rat. Application Notes: RAT IgG1 isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user
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