Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free, Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:5,000
Application Notes:
Application Notes: Anti-Alkaline Phosphatase peroxidase has been tested by dot blot and is functional in ELISA, Western blot, and Immunohistochemical assays. Optimization should be reached by researcher
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